Typical Testing Data/Standard Curve (for reference only)
Mouse Tryptophan-2, 3-dioxygenase ELISA Kit | TDO elisa kit
Mouse Tryptophan-2, 3-dioxygenase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TDO2,3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TDO2,3 antibody and an TDO2,3-HRP conjugate. The assay sample and buffer are incubated together with TDO2,3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TDO2,3 concentration since TDO2,3 from samples and TDO2,3-HRP conjugate compete for the anti-TDO2,3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TDO2,3 from the sample, fewer sites are left to bind TDO2,3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDO2,3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]
Uniprot Description
TDO2: Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin. Belongs to the tryptophan 2,3-dioxygenase family.
Protein type: Amino Acid Metabolism - tryptophan; EC 1.13.11.11; Oxidoreductase
Chromosomal Location of Human Ortholog: 4q31-q32
Cellular Component: cytosol
Molecular Function: amino acid binding; metal ion binding; heme binding; oxygen binding; tryptophan 2,3-dioxygenase activity
Biological Process: tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; tryptophan catabolic process
Research Articles on TDO
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Product Notes
The Mouse TDO tdo2 (Catalog #AAA722857) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722857 ELISA Kit recognizes Mouse TDO. It is sometimes possible for the material contained within the vial of "Tryptophan-2, 3-dioxygenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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