Transcobalamin II Recombinant Protein | TCN2 recombinant protein

Recombinant Transcobalamin II, Macrocytic Anemia (TCN2)

Cat. Num. AAA2033227

• Gene Names: TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Transcobalamin II; Recombinant Transcobalamin II; Macrocytic Anemia (TCN2); TC2; TCN2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: EF CVIPKMDGQL VEKLGQRLLP WMDRLSSEQL NPSIYVGLRL SSMQAGTKEN LYLHNLKLHY QQCLLRSTSS DDNSGCQTKI SGGSLALYLL ALRANCELLG SRKGDRMVSQ LKWFLEDEKK AIGHHHEGHP HTSYYQYGLS ILALCVHRKR VHDSVVGKLL YAVEHDYFTY QGHLSVDTEA MAGLAFTCLE RFNFNSDLRP RITTAIETVR EKILKAQAPE GYFGNIYSTP LALQMLMTSP GVGLGPACLK ARKSLLLSLQ DGAFQNPMMI SQLLPVLNHK TYLNLISPDC QAPRVMLVPA TEDPVHLSEV SVTLKVSSVL PPYERTVSVF AGASLEDVLN RARDLGEFTY GTQASLSGPY LTSVLGKEAG DREYWQLLRV PDTPLLQGIA DYKPKNGETI ELRLVKM
• Sequence Length: 427

• Applicable Applications for TCN2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 49.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Glu19~Met427
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 339205
• NCBI GeneID: 6948
• Molecular Weight: 44,421 Da
• NCBI Official Full Name: transcobalamin II
• NCBI Official Synonym Full Names: transcobalamin 2
• NCBI Official Symbol: TCN2
• NCBI Official Synonym Symbols: II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• NCBI Protein Information: transcobalamin-2
• UniProt Protein Name: Transcobalamin-2
• Protein Family: Transcobalamin
• UniProt Gene Name: TCN2
• UniProt Synonym Gene Names: TC2; TC-2; TC II; TCII
• UniProt Entry Name: TCO2_HUMAN

NCBI Description

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: endosome; extracellular region; extracellular space; lysosomal lumen

Molecular Function: cobalamin binding; metal ion binding

Biological Process: cobalamin metabolic process; cobalamin transport; cobalt ion transport; vitamin metabolic process; water-soluble vitamin metabolic process

Disease: Transcobalamin Ii Deficiency