Canine Transcobalamin II ELISA Kit | TCN2 elisa kit

Canine Transcobalamin II ELISA Kit

Cat. Num. AAA7262285

• Gene Names: TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• Reactivity: Canine
• Synonyms: Transcobalamin II; Canine Transcobalamin II ELISA Kit; TCN2 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of TCN2. No significant cross-reactivity or interference between TCN2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TCN2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 21

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCN2 elisa kit

Intended Uses: This TCN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TCN2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: TCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TCN2 antibody and an TCN2-HRP conjugate. The assay sample and buffer are incubated together with TCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TCN2 concentration since TCN2 from samples and TCN2-HRP conjugate compete for the anti-TCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TCN2 from the sample, fewer sites are left to bind TCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCN2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2952291
• NCBI GeneID: 6948
• Molecular Weight: 44,421 Da
• NCBI Official Full Name: transcobalamin II, partial
• NCBI Official Synonym Full Names: transcobalamin 2
• NCBI Official Symbol: TCN2
• NCBI Official Synonym Symbols: II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• NCBI Protein Information: transcobalamin-2
• UniProt Protein Name: Transcobalamin-2
• Protein Family: Transcobalamin
• UniProt Gene Name: TCN2
• UniProt Synonym Gene Names: TC2; TC-2; TC II; TCII

NCBI Description

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.