Transcobalamin I Recombinant Protein | TCN1 recombinant protein

Recombinant Transcobalamin I (TCN1)

Cat. Num. AAA2031152

• Gene Names: TCN1; HC; TC1; TCI; TC-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Transcobalamin I; Recombinant Transcobalamin I (TCN1); TCN1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; EICEVSE ENYIRLKPLL NTMIQSNYNR GTSAVNVVLS LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL ALGVCRNAEE NLIYDYHLID KLENKFQAEI ENMEAHNGTP LTNYYQLSLD VLALCLFNGN YSTAEVVNHF TPENKNYYFG SQFSVDTGAM AVLALTCVKK SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN TFSTGEAMQA LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV TVLNGSVFLS VMEKAQKMND TIFGFTMEERSWGPYITCIQ GLCANNNDRT YWELLSGGEP LSQGAGSYVV RNGENLEVRW SKY
• Sequence Length: 433

• Applicable Applications for TCN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 49.3kDa
• Accurate Molecular Mass (KD): 45kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu24~Tyr433
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21071008
• NCBI GeneID: 6947
• NCBI Accession #: NP_001053.2
• NCBI GenBank Nucleotide #: NM_001062.3
• Molecular Weight: 48,207 Da
• NCBI Official Full Name: transcobalamin-1
• NCBI Official Synonym Full Names: transcobalamin I (vitamin B12 binding protein, R binder family)
• NCBI Official Symbol: TCN1
• NCBI Official Synonym Symbols: HC; TC1; TCI; TC-1
• NCBI Protein Information: transcobalamin-1
• UniProt Protein Name: Transcobalamin-1
• Protein Family: Transcobalamin
• UniProt Gene Name: TCN1
• UniProt Synonym Gene Names: TC1; TC-1; HC; TC I; TCI
• UniProt Entry Name: TCO1_HUMAN

NCBI Description

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This protein is a major constituent of secondary granules in neutrophils and facilitates the transport of cobalamin into cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TCN1: Vitamin B12-binding protein. Transports cobalamin into cells. Belongs to the eukaryotic cobalamin transport proteins family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q11-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process