Human Transcription factor 4 ELISA Kit | TCF4 elisa kit

Human Transcription factor 4 ELISA Kit

Cat. Num. AAA762503

• Gene Names: TCF4; TCF7L2
• Reactivity: Human
• Synonyms: Transcription factor 4; Human Transcription factor 4 ELISA Kit; TCF4/Transcription factor 4/TCF-4/SL3-3 enhancer factor 2/SEF-2/Class B basic helix-loop-helix protein 19/bHLHb19/Immunoglobulin transcription factor 2/ITF-2/E2-2/ITF2/PTHS/SEF2/SEF2-1/SEF2-1A/SEF2-1B/bHLHb19; TCF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TAT. No significant cross-reactivity or interference between TAT and analogues was observed.
• Sequence Length: 642

• Samples: Plasma
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TAT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TAT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCF4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TAT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TAT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TAT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TAT can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 50979072
• NCBI GeneID: 403949
• NCBI Accession #: NP_001003268.1
• NCBI GenBank Nucleotide #: NM_001003268.1
• Molecular Weight: 68,355 Da
• NCBI Official Full Name: transcription factor 4
• NCBI Official Symbol: TCF4
• NCBI Official Synonym Symbols: TCF7L2
• NCBI Protein Information: transcription factor 4
• UniProt Protein Name: Transcription factor 4
• Protein Family: Transcription factor
• UniProt Gene Name: TCF4
• UniProt Synonym Gene Names: ITF2; TFE; TCF-4; ITF-2
• UniProt Entry Name: ITF2_CANLF

Uniprot Description

Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (). Binds to the thyroglobulin promoter.