Elongin B Recombinant Protein | ELOB recombinant protein

Recombinant Elongin B (ELOB)

Cat. Num. AAA2034585

• Gene Names: TCEB2; ELOB; SIII
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Elongin B; Recombinant Elongin B (ELOB); Transcription Elongation Factor B(SIII)Polypeptide 2; RNA polymerase II transcription factor SIII subunit B; Elongin 18 kDa subunit; ELOB recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Human
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 72

• Applicable Applications for ELOB recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Met1~Gln118
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Met1~Gln118 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2996649
• NCBI GeneID: 6923
• Molecular Weight: Predicted: 16.8kDa
• NCBI Official Full Name: elongin B, partial
• NCBI Official Synonym Full Names: transcription elongation factor B subunit 2
• NCBI Official Symbol: TCEB2
• NCBI Official Synonym Symbols: ELOB; SIII
• NCBI Protein Information: transcription elongation factor B polypeptide 2
• UniProt Protein Name: Transcription elongation factor B polypeptide 2
• UniProt Gene Name: TCEB2
• UniProt Synonym Gene Names: EloB
• UniProt Entry Name: ELOB_HUMAN

NCBI Description

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]

Uniprot Description

TCEB2: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: Cul2-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex; cytosol; nucleoplasm; VCB complex

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: protein complex assembly; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter