SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Elob recombinant protein
Recombinant Elob
Gene Names
Elob; Tceb2; 0610040H15Rik
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
Elob; Recombinant Elob; Elongin B; 0610040H15Rik; Tceb2; Elob recombinant protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 1mM DTT, 10% Glycerol.
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-118aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q
Sequence Length
118
Applicable Applications for Elob recombinant protein
SDS-PAGE
Species
Mouse
Tag
His-Tag
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for Elob recombinant protein
Elob, also known as elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimeric consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant mouse Elob, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for Elob recombinant protein
References
Piessevaux J., et al. (2008) J Biol Chem. 283(31):21334-46.;Duan DR., et al. (1995) Science. 269(5229):1402-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15.6 kDa (141 aa), confirmed by MALDI-TOF
NCBI Official Full Name
elongin-B
NCBI Official Synonym Full Names
elongin B
NCBI Official Symbol
Elob
NCBI Official Synonym Symbols
Tceb2; 0610040H15Rik
NCBI Protein Information
elongin-B
UniProt Protein Name
Transcription elongation factor B polypeptide 2
UniProt Gene Name
Tceb2
UniProt Synonym Gene Names
EloB
UniProt Entry Name
ELOB_MOUSE
Research Articles on Elob
Similar Products
Product Notes
The Elob tceb2 (Catalog #AAA201080) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-118aa. AAA Biotech's Elob can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Elob tceb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q. It is sometimes possible for the material contained within the vial of "Elob, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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