Transcription Elongation Factor A2 Recombinant Protein | TCEA2 recombinant protein

Recombinant Transcription Elongation Factor A2 (TCEA2)

Cat. Num. AAA2034587

• Gene Names: TCEA2; TFIIS
• Reactivity: Mouse
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Transcription Elongation Factor A2; Recombinant Transcription Elongation Factor A2 (TCEA2); Transcription elongation factor S-II protein 2; Testis-specific S-II; TCEA2 recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Mouse
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 272

• Applicable Applications for TCEA2 recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Met1~Ile218
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Met1~Ile218 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 38505156
• NCBI GeneID: 6919
• NCBI Accession #: NP_942016.1
• NCBI GenBank Nucleotide #: NM_198723.1
• Molecular Weight: Predicted: 28.0kDa
• NCBI Official Full Name: transcription elongation factor A protein 2 isoform b
• NCBI Official Synonym Full Names: transcription elongation factor A2
• NCBI Official Symbol: TCEA2
• NCBI Official Synonym Symbols: TFIIS
• NCBI Protein Information: transcription elongation factor A protein 2
• UniProt Protein Name: Transcription elongation factor A protein 2
• Protein Family: Tuliposide A-converting enzyme
• UniProt Gene Name: TCEA2
• UniProt Entry Name: TCEA2_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TCEA2: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Belongs to the TFS-II family.

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: centrosome; nucleoplasm

Molecular Function: protein binding