Rabbit anti-Human TAS2R3 Polyclonal Antibody | anti-TAS2R3 antibody

TAS2R3, CT (TAS2R3, Taste receptor type 2 member 3) (PE)

Cat. Num. AAA6353698

• Gene Names: TAS2R3; T2R3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAS2R3; Polyclonal Antibody; CT (TAS2R3; Taste receptor type 2 member 3) (PE); Taste receptor type 2 member 3; anti-TAS2R3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TAS2R3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAS2R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-313 amino acids from the C-terminal region of human TAS2R3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAS2R3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TAS2R3 antibody detected the TAS2R3 protein (arrow).)

Related Product Information for anti-TAS2R3 antibody

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless taste receptor genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.

Product Categories/Family for anti-TAS2R3 antibody

Antibodies; Receptors

References

Calvo, S.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7507-7512(2009). Go, Y., et al. Genetics 170(1):313-326(2005). Fischer, A., et al. Mol. Biol. Evol. 22(3):432-436(2005). Zhang, Y., et al. Cell 112(3):293-301(2003). Montmayeur, J.P., et al. Curr. Opin. Neurobiol. 12(4):366-371(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 8394398
• NCBI GeneID: 50831
• NCBI Accession #: NP_058639.1
• NCBI GenBank Nucleotide #: NM_016943.2
• UniProt Accession #: Q9NYW6; Q645W2; Q75MV6; A4D1U2
• Molecular Weight: 35,915 Da
• NCBI Official Full Name: taste receptor type 2 member 3
• NCBI Official Synonym Full Names: taste receptor, type 2, member 3
• NCBI Official Symbol: TAS2R3
• NCBI Official Synonym Symbols: T2R3
• NCBI Protein Information: taste receptor type 2 member 3; candidate taste receptor T2R3
• UniProt Protein Name: Taste receptor type 2 member 3
• Protein Family: Taste receptor
• UniProt Gene Name: TAS2R3
• UniProt Synonym Gene Names: T2R3
• UniProt Entry Name: TA2R3_HUMAN

NCBI Description

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless taste receptor genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. Ref.1 Ref.7

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in the antrum and fundus (part of the stomach), duodenum and in gastric endocrine cells. Ref.1

Miscellaneous: Several bitter taste receptors are expressed in a single taste receptor cell.

Sequence similarities: Belongs to the G-protein coupled receptor T2R family.

Sequence caution: The sequence AAU21157.1 differs from that shown. Reason: Erroneous initiation.