Tyrosine Aminotransferase Recombinant Protein | TAT recombinant protein

Recombinant Tyrosine Aminotransferase (TAT)

Cat. Num. AAA2034348

• Reactivity: Human
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Tyrosine Aminotransferase; Recombinant Tyrosine Aminotransferase (TAT); Tyrosine Transaminase; L-tyrosine:2-oxoglutarate aminotransferase; TAT recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >95%
• Sequence Length: 454

• Applicable Applications for TAT recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Cys221~Lys454
• Tag: Prokaryotic expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4507369
• NCBI GeneID: 6898
• NCBI Accession #: NP_000344.1
• NCBI GenBank Nucleotide #: NM_000353.2
• Molecular Weight: 30.0kDa
• NCBI Official Full Name: tyrosine aminotransferase
• NCBI Official Synonym Full Names: tyrosine aminotransferase
• NCBI Official Symbol: TAT
• NCBI Protein Information: tyrosine aminotransferase
• UniProt Protein Name: Tyrosine aminotransferase
• Protein Family: Tyrosine aminotransferase
• UniProt Gene Name: TAT
• UniProt Synonym Gene Names: TAT
• UniProt Entry Name: ATTY_HUMAN

NCBI Description

This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]

Uniprot Description

TAT: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine. Defects in TAT are the cause of tyrosinemia type 2 (TYRO2); also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - phenylalanine; Mitochondrial; EC 2.6.1.5; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; Transferase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol; mitochondrion

Molecular Function: amino acid binding; pyridoxal phosphate binding; tyrosine transaminase activity

Biological Process: 2-oxoglutarate metabolic process; biosynthetic process; glutamate metabolic process; L-phenylalanine catabolic process; response to glucocorticoid stimulus; response to mercury ion; response to oxidative stress; tyrosine catabolic process

Disease: Tyrosinemia, Type Ii