Human TAR DNA binding protein ELISA Kit | TARDBP elisa kit

Human TAR DNA-binding protein 43, TARDBP/TDP43 ELISA Kit

Cat. Num. AAA705899

• Gene Names: TARDBP; ALS10; TDP-43
• Reactivity: Human
• Synonyms: TAR DNA binding protein; Human TAR DNA-binding protein 43; TARDBP/TDP43 ELISA Kit; Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA kit; RP4-635E18.2; ALS10; TDP-43; TAR DNA-binding protein-43; TARDBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TARDBP. No significant cross-reactivity or interference between human TARDBP and analogues was observed.
• Sequence Length: 414

• Samples: Serum, plasma, cell culture supernates, cerebrospinal fluid (csf)
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TARDBP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARDBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARDBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARDBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARDBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6678271
• NCBI GeneID: 23435
• NCBI Accession #: NP_031401.1
• NCBI GenBank Nucleotide #: NM_007375.3
• UniProt Accession #: Q13148; Q53H27; Q6FI92; Q96DJ0; A4GUK4; A4GUK5; A4GUK6; B2R629; E2PU12
• Molecular Weight: 44,740 Da
• NCBI Official Full Name: TAR DNA-binding protein 43
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: TARDBP
• NCBI Official Synonym Symbols: ALS10; TDP-43
• NCBI Protein Information: TAR DNA-binding protein 43; TAR DNA-binding protein-43
• UniProt Protein Name: TAR DNA-binding protein 43
• Protein Family: TAR DNA-binding protein
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43
• UniProt Entry Name: TADBP_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.2 PublicationsManual assertion based on experiment in:Ref.2