Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
EU-Taq DNA Taq DNA Polymerase
EU-Taq DNA Polynerase
Multiplexed genotyping
Quantitative Real-time PCR
Nested PCR
B: 10x PCR buffer (no Mg2+); 1 vial (1.5ml)
C: 100mM MgCl2; 1 vial (1 ml).
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
Testing Data
(Figure A, Amplification of genomic DNA; using EU-taq with various primer set. PCR; conditions: 9 4 degree C x 2'; 9 4 degree C x 45", 55 oC x; 45", 72 oC x 30"-3', for 30 cycles. Figure B, Duplex PCR amplification. Lane1,; allele specific genotyping of human PAX6; mutation, 490bp-mutant band, 320bp-wild; type band; Lane2, two primer sets targeted; to ALDH3 gene (200bp) and SHH (Sonic; Hedgehog) gene. PCR conditions: 9 4 degree C x; 2'; 9 4 degree C x 30", 55 oC x 30", 72 oC x 30", for; 30 cycles. Figure C and D, Titration of enzyme; concentration in 25 mul PCR reaction (C, EUtaq,; D, Taq from other company). Lane M,; 100-bp maker, lane1, 0.01 U; lane2, 0.02 U;; lane3, 0.1 U; lane4, 0.2 U; lane5, 0.5 U;; lane7, 1 U.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 68 oC x 30", for 25 cycles. Figure E, Titration of plasmid DNA; concentration in 25 mul PCR reaction. Lane; M, 100-bp marker, lane1, 1 ng; lane2, 0.1; ng; lane3, 0.01 ng; lane4, 5 pg; lane5, 1 pg;; lane6, 0.5 pg; lane7, 0.1 pg; lane8, 0.01 pg.; PCR conditions: 9 4 degree C x 2'; 9 4 degree C x 30", 55; oC x 30", 72 oC x 30", for 25 cycles.)
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Product Notes
The EU-Taq DNA (Catalog #AAA433677) is a Taq DNA Polymerase and is intended for research purposes only. The product is available for immediate purchase. High-throughput genotyping/screeningMultiplexed genotyping
Quantitative Real-time PCR
Nested PCR. Researchers should empirically determine the suitability of the EU-Taq DNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EU-Taq DNA, Taq DNA Polymerase" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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