TATA Box Binding Protein Associated Factor 13 (TAF13) Recombinant Protein | TAF13 recombinant protein

Recombinant TATA Box Binding Protein Associated Factor 13 (TAF13)

Cat. Num. AAA2011052

• Gene Names: TAF13; TAF2K; TAFII18; TAFII-18; TAF(II)18
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: TATA Box Binding Protein Associated Factor 13 (TAF13); Recombinant TATA Box Binding Protein Associated Factor 13 (TAF13); TAF13 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MADEEEDPTF EEENEEIGGG AEGGQGKRKR LFSKELRCMM YGFGDDQNPY TESVDILEDL VIEFITEMTH KAMSIGRQGR VQVEDIVFLI RKDPRKFARV KDLLTMNEEL KRARKAFDEA NYGS
• Sequence Length: 124

• Applicable Applications for TAF13 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser124 (Accession # Q15543) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5032155
• NCBI GeneID: 6884
• NCBI Accession #: NP_005636.1
• NCBI GenBank Nucleotide #: NM_005645.3
• UniProt Accession #: Q15543; Q5TYV6; B2R5E5
• Molecular Weight: 18.1kDa
• NCBI Official Full Name: transcription initiation factor TFIID subunit 13
• NCBI Official Synonym Full Names: TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 18kDa
• NCBI Official Symbol: TAF13
• NCBI Official Synonym Symbols: TAF2K; TAFII18; TAFII-18; TAF(II)18
• NCBI Protein Information: transcription initiation factor TFIID subunit 13; transcription initiation factor TFIID 18 kD subunit; transcription initiation factor TFIID 18 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, K, 18kD
• UniProt Protein Name: Transcription initiation factor TFIID subunit 13
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF13
• UniProt Synonym Gene Names: TAF2K; TAFII18; TAF(II)18; TAFII-18; TAFII18
• UniProt Entry Name: TAF13_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit associated with a subset of TFIID complexes. This subunit interacts with TBP and with two other small subunits of TFIID, TAF10 and TAF11. There is a pseudogene located on chromosome 6. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF13: a TBP-associated factor (TAF). A component of TFIID complexes composed of the TATA binding protein (TBP), TAF10 and TAF11. TAF13 and TAF10 contain non-canonical histone folds, forming a histone-like pair in TFIID complexes.

Protein type: Translation

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; transcription factor TFIID complex; nucleolus; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription cofactor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription initiation; RNA elongation from RNA polymerase II promoter; gene expression