YWHAG sirna

YWHAG siRNA (Rat)

Cat. Num. AAA8232207

• Gene Names: Ywhag; 14-3-3G
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YWHAG; YWHAG siRNA (Rat); 14-3-3 protein gamma; YWHAG sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: YWHAG siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 247

• Applicable Applications for YWHAG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat YWHAG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YWHAG sirna

siRNA to inhibit YWHAG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9507245
• NCBI GeneID: 56010
• NCBI Accession #: NP_062249.1
• NCBI GenBank Nucleotide #: NM_019376.2
• UniProt Accession #: P61983; O70457; P35214; Q9UDP2; Q9UN99; A0JPM0
• Molecular Weight: 28,303 Da
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
• NCBI Official Symbol: Ywhag
• NCBI Official Synonym Symbols: 14-3-3G
• NCBI Protein Information: 14-3-3 protein gamma
• UniProt Protein Name: 14-3-3 protein gamma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: Ywhag
• UniProt Entry Name: 1433G_RAT

NCBI Description

belongs to the 14-3-3 family of proteins; mediates signal transduction via activation of protein kinase C and calcium/calmodulin-dependent protein kinase II [RGD, Feb 2006]

Uniprot Description

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; myelin sheath

Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding; actin binding; enzyme regulator activity

Biological Process: cellular response to insulin stimulus; regulation of neuron differentiation; regulation of catalytic activity; regulation of synaptic plasticity; signal transduction; protein targeting