YBX1 sirna

YBX1 siRNA (Rat)

Cat. Num. AAA8209884

• Gene Names: Ybx1; Yb1; Byb1; Cbfa; Dbpb; Ef1a; Msy1; Nsep1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YBX1; YBX1 siRNA (Rat); MSY-1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1; YBX1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: YBX1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 322

• Applicable Applications for YBX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat YBX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YBX1 sirna

siRNA to inhibit YBX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 92373398
• NCBI GeneID: 500538
• NCBI Accession #: NP_113751.3
• NCBI GenBank Nucleotide #: NM_031563.3
• UniProt Accession #: P62961; P22568; P27817; P43482; Q4KMA4
• Molecular Weight: 35,730 Da
• NCBI Official Full Name: nuclease-sensitive element-binding protein 1
• NCBI Official Synonym Full Names: Y box binding protein 1
• NCBI Official Symbol: Ybx1
• NCBI Official Synonym Symbols: Yb1; Byb1; Cbfa; Dbpb; Ef1a; Msy1; Nsep1
• NCBI Protein Information: nuclease-sensitive element-binding protein 1
• UniProt Protein Name: Nuclease-sensitive element-binding protein 1
• Protein Family: Nuclease-sensitive element-binding protein
• UniProt Gene Name: Ybx1
• UniProt Synonym Gene Names: Msy-1; Nsep1; Yb1; CBF-A; DBPB; EFI-A; YB-1
• UniProt Entry Name: YBOX1_RAT

NCBI Description

transcription factor; acts as a regulator of protein tyrosine phosphatase PTP1B expression [RGD, Apr 2006]

Uniprot Description

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.

Protein type: RNA processing; RNA splicing; RNA-binding; Transcription factor

Cellular Component: nuclear membrane; intracellular membrane-bound organelle; stress granule; perinuclear region of cytoplasm; dendrite; cytoplasm; extracellular region; ribonucleoprotein complex; nucleus; U12-dependent spliceosome

Molecular Function: mRNA binding; DNA binding; p53 binding; sequence-specific DNA binding; RNA binding; chromatin binding; transcription factor binding; single-stranded DNA binding

Biological Process: negative regulation of striated muscle cell differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; in utero embryonic development; RNA splicing; positive regulation of cell division; positive regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; mRNA processing; negative regulation of apoptosis