VPS4A sirna

VPS4A siRNA (Rat)

Cat. Num. AAA8203970

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VPS4A; VPS4A siRNA (Rat); VPS4-A; Vacuolar protein sorting-associated protein 4A; VPS4A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: VPS4A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for VPS4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VPS4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VPS4A sirna

siRNA to inhibit VPS4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21728408
• NCBI GeneID: 246772
• NCBI Accession #: NP_663711.1
• NCBI GenBank Nucleotide #: NM_145678.1
• UniProt Accession #: Q793F9
• Molecular Weight: 48,907 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 4A
• NCBI Official Synonym Full Names: vacuolar protein sorting 4 homolog A (S. cerevisiae)
• NCBI Official Symbol: Vps4a
• NCBI Protein Information: vacuolar protein sorting-associated protein 4A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps4a
• UniProt Synonym Gene Names: vps4-A
• UniProt Entry Name: VPS4A_RAT

NCBI Description

binds Rho family GTPase Rnd2; may play a role in endosomal trafficking [RGD, Feb 2006]

Uniprot Description

VPS4A: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis. Belongs to the AAA ATPase family.

Protein type: Vesicle; EC 3.6.4.6

Cellular Component: spindle pole; centrosome; late endosome membrane; perinuclear region of cytoplasm; early endosome; cytoplasm; midbody; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; Rho GTPase binding; microtubule-severing ATPase activity; ATP binding

Biological Process: cell separation during cytokinesis; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; metabolic process; endosome transport; mitotic metaphase plate congression; negative regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; vesicle-mediated transport; protein transport; cell division; abscission; nuclear organization and biogenesis; vacuole organization and biogenesis