TRPV1 sirna

TRPV1 siRNA (Rat)

Cat. Num. AAA8215212

• Gene Names: Trpv1; Vr1; Vr1l1; VR.5'sv; TRPV1_SON
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPV1; TRPV1 siRNA (Rat); VR1; VR1L; Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; Vanilloid receptor type 1-like; TRPV1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TRPV1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 838

• Applicable Applications for TRPV1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TRPV1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPV1 sirna

siRNA to inhibit TRPV1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14010883
• NCBI GeneID: 83810
• NCBI Accession #: NP_114188.1
• NCBI GenBank Nucleotide #: NM_031982.1
• UniProt Accession #: O35433; Q920B3; Q920B4; Q9JLM0; Q9JM56; Q9JM57
• Molecular Weight: 54,420 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily V, member 1
• NCBI Official Symbol: Trpv1
• NCBI Official Synonym Symbols: Vr1; Vr1l1; VR.5'sv; TRPV1_SON
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 1
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpv1
• UniProt Synonym Gene Names: Vr1; Vr1l; TrpV1; OTRPC1
• UniProt Entry Name: TRPV1_RAT

NCBI Description

ion channel that demonstrates heat-evoked membrane currents [RGD, Feb 2006]

Uniprot Description

TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.

Protein type: Channel, cation; Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: postsynaptic membrane; neuron projection; cell soma; membrane; dendrite; integral to membrane; plasma membrane; synapse; cell junction; cytosol; intrinsic to plasma membrane; external side of plasma membrane

Molecular Function: identical protein binding; excitatory extracellular ligand-gated ion channel activity; calcium channel activity; ion channel activity; phosphoprotein binding; ligand-gated ion channel activity; calmodulin binding; protein binding; transmembrane receptor activity; chloride channel regulator activity; cation transmembrane transporter activity; calcium-release channel activity; cation channel activity; ATP binding

Biological Process: urinary bladder smooth muscle contraction; response to peptide hormone stimulus; diet induced thermogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; microglial cell activation; response to pain; thermoregulation; sensory perception of mechanical stimulus; thermoception; sensory perception of pain; fever; signal transduction; behavioral response to pain; elevation of cytosolic calcium ion concentration; calcium ion transport; inflammatory response; peptide secretion; detection of chemical stimulus involved in sensory perception of pain; response to organic nitrogen; glutamate secretion; response to heat; smooth muscle contraction involved in micturition; lipid metabolic process; response to pH