TRAF6 sirna

TRAF6 siRNA (Rat)

Cat. Num. AAA8229937

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRAF6; TRAF6 siRNA (Rat); TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; TRAF6 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TRAF6 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 530

• Applicable Applications for TRAF6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TRAF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for TRAF6 sirna

siRNA to inhibit TRAF6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157820501
• NCBI GeneID: 311245
• NCBI Accession #: NP_001101224.1
• NCBI GenBank Nucleotide #: NM_001107754.2
• UniProt Accession #: B5DF45
• Molecular Weight: 60,253 Da
• NCBI Official Full Name: TNF receptor-associated factor 6
• NCBI Official Synonym Full Names: TNF receptor-associated factor 6, E3 ubiquitin protein ligase
• NCBI Official Symbol: Traf6
• NCBI Protein Information: TNF receptor-associated factor 6
• UniProt Protein Name: TNF receptor-associated factor 6
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: Traf6
• UniProt Entry Name: TRAF6_RAT

Uniprot Description

TRAF6: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF- kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL- 1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Homotrimer. Homooligomer. N-terminal region is dimeric while C-terminal region is trimeric; maybe providing a mode of oligomerization. Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, TRAF, TRAF-interacting protein TRIP and TNF receptor associated protein TDP2. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ. Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675. Interacts with AJUBA. Interacts with TICAM1 and TICAM2. Interacts with ZFAND5. Interacts with ARRB1 and ARRB2. Interacts with MAP3K7 and TAB1/MAP3K7IP1; during IL-1 signaling. Interacts with UBE2N. Interacts with TGFBR1, HDAC1 and RANGAP1. Interacts with AKT1, AKT2 and AKT3. Interacts (via TRAF domains) with NUMBL (via C-terminal). Interacts (via TRAF domains) with WDR34 (via WD domains). Interacts with RBCK1. Interacts with TRAF3IP2. Interacts with LIMD1 (via LIM domains). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Belongs to the TNF receptor-associated factor family. A subfamily.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; Ligase

Cellular Component: internal side of plasma membrane; protein complex; mitochondrion; perinuclear region of cytoplasm; cytoplasm; nucleolus; plasma membrane; lipid particle; cell cortex; intracellular; nucleus; cytosol

Molecular Function: protein kinase B binding; ubiquitin conjugating enzyme binding; zinc ion binding; histone deacetylase binding; ubiquitin protein ligase binding; thioesterase binding; ubiquitin-protein ligase activity; protein N-terminus binding; mitogen-activated protein kinase kinase kinase binding; tumor necrosis factor receptor binding; protein kinase binding; ligase activity

Biological Process: positive regulation of JNK activity; I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; positive regulation of osteoclast differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; positive regulation of lipopolysaccharide-mediated signaling pathway; protein ubiquitination; positive regulation of T cell cytokine production; antigen processing and presentation of exogenous peptide antigen via MHC class II; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; signal transduction; bone remodeling; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; JNK cascade; positive regulation of T cell proliferation; protein complex assembly; regulation of immunoglobulin secretion; bone resorption; protein autoubiquitination; T-helper 1 type immune response; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; activation of NF-kappaB-inducing kinase; odontogenesis of dentine-containing teeth; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; neural tube closure; positive regulation of interleukin-2 production; activation of protein kinase activity; immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell development