TRA2B sirna

TRA2B siRNA (Rat)

Cat. Num. AAA829539

• Gene Names: Tra2b; Sfrs10; Srsf10
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRA2B; TRA2B siRNA (Rat); SFRS10; Transformer-2 protein homolog beta; TRA-2 beta; TRA2-beta; RA301; Splicing factor. arginine/serine-rich 10; Transformer-2 protein homolog B; TRA2B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TRA2B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 288

• Applicable Applications for TRA2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TRA2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRA2B sirna

siRNA to inhibit TRA2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16923966
• NCBI GeneID: 117259
• NCBI Accession #: NP_476460.1
• NCBI GenBank Nucleotide #: NM_057119.1
• UniProt Accession #: P62997; O15449; Q15815; Q64283
• Molecular Weight: 33,666 Da
• NCBI Official Full Name: transformer-2 protein homolog beta
• NCBI Official Synonym Full Names: transformer 2 beta homolog (Drosophila)
• NCBI Official Symbol: Tra2b
• NCBI Official Synonym Symbols: Sfrs10; Srsf10
• NCBI Protein Information: transformer-2 protein homolog beta
• UniProt Protein Name: Transformer-2 protein homolog beta
• Protein Family: Transformer-2 protein
• UniProt Gene Name: Tra2b
• UniProt Synonym Gene Names: Sfrs10; TRA-2 beta; TRA2-beta
• UniProt Entry Name: TRA2B_RAT

NCBI Description

may act as an RNA splicing factor; may play a role in response to reactive oxygen species [RGD, Feb 2006]

Uniprot Description

TRA2B: Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre- mRNA. Belongs to the splicing factor SR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Spliceosome

Cellular Component: nucleus

Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; response to reactive oxygen species; cerebral cortex regionalization; regulation of alternative nuclear mRNA splicing, via spliceosome