TNFAIP1 sirna

TNFAIP1 siRNA (Rat)

Cat. Num. AAA828833

• Gene Names: Tnfaip1; Edp1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNFAIP1; TNFAIP1 siRNA (Rat); BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; Tumor necrosis factor-induced protein 1; TNFAIP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TNFAIP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 316

• Applicable Applications for TNFAIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TNFAIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNFAIP1 sirna

siRNA to inhibit TNFAIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33636730
• NCBI GeneID: 287543
• NCBI Accession #: NP_891995.1
• NCBI GenBank Nucleotide #: NM_182950.4
• UniProt Accession #: Q7TNY1
• Molecular Weight: 36,046 Da
• NCBI Official Full Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• NCBI Official Symbol: Tnfaip1
• NCBI Official Synonym Symbols: Edp1
• NCBI Protein Information: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• UniProt Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Protein Family: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein
• UniProt Gene Name: Tnfaip1
• UniProt Entry Name: BACD2_RAT

Uniprot Description

TNFAIP1: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. Belongs to the BACURD family.

Protein type: DNA replication; Ubiquitin conjugating system

Cellular Component: cytoplasm; nucleolus; endosome

Molecular Function: protein domain specific binding; protein binding; ubiquitin-protein ligase activity; GTP-Rho binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell migration; protein heterooligomerization; protein ubiquitination; DNA repair; negative regulation of Rho protein signal transduction; regulation of transcription, DNA-dependent; stress fiber formation; immune response; actin cytoskeleton organization and biogenesis; DNA replication; positive regulation of DNA replication; protein homooligomerization