ST6GAL1 sirna

ST6GAL1 siRNA (Rat)

Cat. Num. AAA8200666

• Gene Names: St6gal1; Siat1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ST6GAL1; ST6GAL1 siRNA (Rat); SIAT1; Beta-galactoside alpha-2.6-sialyltransferase 1; Alpha 2.6-ST 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2.6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1; ST6GAL1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ST6GAL1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 403

• Applicable Applications for ST6GAL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ST6GAL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ST6GAL1 sirna

siRNA to inhibit ST6GAL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164519128
• NCBI GeneID: 25197
• NCBI Accession #: NP_001106815.1
• NCBI GenBank Nucleotide #: NM_001113344.1
• UniProt Accession #: P13721
• Molecular Weight: 21,874 Da
• NCBI Official Full Name: beta-galactoside alpha-2,6-sialyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• NCBI Official Symbol: St6gal1
• NCBI Official Synonym Symbols: Siat1
• NCBI Protein Information: beta-galactoside alpha-2,6-sialyltransferase 1
• UniProt Protein Name: Beta-galactoside alpha-2,6-sialyltransferase 1
• Protein Family: Beta-galactoside alpha-2,6-sialyltransferase
• UniProt Gene Name: St6gal1
• UniProt Synonym Gene Names: Siat1; Alpha 2,6-ST 1; ST6GalI
• UniProt Entry Name: SIAT1_RAT

NCBI Description

may be involved in cell-type specific terminal glycosylation [RGD, Feb 2006]

Uniprot Description

SIAT1: a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. Is normally found in the trans cisternae of the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found.

Protein type: Glycan Metabolism - N-glycan biosynthesis; Transferase; EC 2.4.99.1; Membrane protein, integral

Cellular Component: Golgi apparatus; extracellular region; integral to membrane; integral to Golgi membrane

Molecular Function: sialyltransferase activity; beta-galactoside alpha-2,6-sialyltransferase activity

Biological Process: protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; N-acetylneuraminate metabolic process