Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: SAMHD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 64, 69 kDa Observed band size: 73 kDa)
Rabbit anti-Human SAMHD1 Polyclonal Antibody | anti-SAMHD1 antibody
SAMHD1 Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Antigen Affinity purified & Affinity purified
Synonyms
SAMHD1; Polyclonal Antibody; SAMHD1 Antibody; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (dNTPase) (EC 3.1.5.-) (Dendritic cell-derived IFNG-induced protein) (DCIP) (Monocyte protein 5) (MOP-5) (SAM domain and HD domain-containing protein 1); MOP5; anti-SAMHD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Antigen Affinity purified & Affinity purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH7.4
Applicable Applications for anti-SAMHD1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:5000
IHC: 1:100-1:500
IHC: 1:100-1:500
Conjugation
Non-conjugated
Immunogen
Recombinant Human Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 protein (1-314AA)
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: SAMHD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 64, 69 kDa Observed band size: 73 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: SAMHD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 64, 69 kDa Observed band size: 73 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Related Product Information for anti-SAMHD1 antibody
Host restriction nuclease involved in defense response to virus. Has dNTPase activity and reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur. Blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May play a role in mediating proinflammatory responses to TNF-alpha signaling (PubMed:18546154, PubMed:19525956, PubMed:22056990, PubMed:24336198, PubMed:28229507). Has ribonuclease activity, acting on single-stranded RNA. This activity is essential for H1V-1 restriction (PubMed:25038827).
Product Categories/Family for anti-SAMHD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 65; 69 kDa
Observed: 62 kDa
Observed: 62 kDa
NCBI Official Full Name
deoxynucleoside triphosphate triphosphohydrolase SAMHD1
NCBI Official Synonym Full Names
SAM domain and HD domain 1
NCBI Official Symbol
SAMHD1
NCBI Official Synonym Symbols
DCIP; CHBL2; HDDC1; MOP-5; SBBI88
NCBI Protein Information
deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; monocyte protein 5; dendritic cell-derived IFNG-induced protein; SAM domain and HD domain-containing protein 1
UniProt Protein Name
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein Family
UniProt Gene Name
SAMHD1
UniProt Synonym Gene Names
MOP5; dNTPase; DCIP; MOP-5
UniProt Entry Name
SAMH1_HUMAN
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Product Notes
The SAMHD1 samhd1 (Catalog #AAA7136172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAMHD1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAMHD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:100-1:500. Researchers should empirically determine the suitability of the SAMHD1 samhd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAMHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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