SPATA18 sirna

SPATA18 siRNA (Rat)

Cat. Num. AAA8203890

• Gene Names: Spata18; spetex-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPATA18; SPATA18 siRNA (Rat); MIEAP; SPETEX1; Mitochondria-eating protein; Spermatid-expressing gene 1 protein; Spetex-1; Spermatogenesis-associated protein 18; SPATA18 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SPATA18 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 568

• Applicable Applications for SPATA18 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SPATA18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPATA18 sirna

siRNA to inhibit SPATA18 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51491896
• NCBI GeneID: 289586
• NCBI Accession #: NP_955406.2
• NCBI GenBank Nucleotide #: NM_199374.2
• UniProt Accession #: Q6AYL6; Q764P0; D3ZEU0
• Molecular Weight: 62,633 Da
• NCBI Official Full Name: mitochondria-eating protein
• NCBI Official Synonym Full Names: spermatogenesis associated 18
• NCBI Official Symbol: Spata18
• NCBI Official Synonym Symbols: spetex-1
• NCBI Protein Information: mitochondria-eating protein
• UniProt Protein Name: Mitochondria-eating protein
• Protein Family: Mitochondria-eating protein
• UniProt Gene Name: Spata18
• UniProt Synonym Gene Names: Mieap; Spetex1; Spetex-1
• UniProt Entry Name: MIEAP_RAT

NCBI Description

may play a role in differentiation of late elongate spermatids to mature spermatozoa [RGD, Feb 2006]

Uniprot Description

SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; cytoplasm

Biological Process: multicellular organismal development; spermatogenesis; cell differentiation; response to DNA damage stimulus