Mouse CHD3 Monoclonal Antibody | anti-CHD3 antibody

CHD3 (Chromodomain Helicase DNA Binding Protein 3, Mi-2a, Mi2-alpha, ZFH)

Cat. Num. AAA603012

• Gene Names: CHD3; ZFH; Mi-2a; Mi2-ALPHA
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CHD3; Monoclonal Antibody; CHD3 (Chromodomain Helicase DNA Binding Protein 3; Mi-2a; Mi2-alpha; ZFH); Anti -CHD3 (Chromodomain Helicase DNA Binding Protein 3; anti-CHD3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C72
• Specificity: Recognizes human CHD3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-CHD3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human CHD3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-CHD3 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 158420731
• NCBI GeneID: 1107
• NCBI Accession #: NP_001005271.2
• NCBI GenBank Nucleotide #: NM_001005271.2
• UniProt Accession #: Q12873; Q9Y4I0; D3DTQ9; Q2TAZ1
• Molecular Weight: 226,592 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 3 isoform 3
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 3
• NCBI Official Symbol: CHD3
• NCBI Official Synonym Symbols: ZFH; Mi-2a; Mi2-ALPHA
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 3; hZFH; CHD-3; OTTHUMP00000197335; OTTHUMP00000197336; OTTHUMP00000197337; zinc finger helicase; ATP-dependent helicase CHD3; mi-2 autoantigen 240 kDa protein; zinc-finger helicase (Snf2-like)
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 3
• Protein Family: CHD3-type chromatin-remodeling factor
• UniProt Gene Name: CHD3
• UniProt Entry Name: CHD3_HUMAN

NCBI Description

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Uniprot Description

CHD-3 iso3: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity. Widely expressed. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; centrosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; helicase activity; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; centrosome organization and biogenesis; chromatin modification; spindle organization and biogenesis; DNA duplex unwinding