SNAI2 sirna

SNAI2 siRNA (Rat)

Cat. Num. AAA8232328

• Gene Names: Snai2; Slug
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNAI2; SNAI2 siRNA (Rat); SLUG; SLUGH; Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2; SNAI2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SNAI2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 268

• Applicable Applications for SNAI2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SNAI2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNAI2 sirna

siRNA to inhibit SNAI2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 29789044
• NCBI GeneID: 25554
• NCBI Accession #: NP_037167.1
• NCBI GenBank Nucleotide #: NM_013035.1
• UniProt Accession #: O08954; Q8R482
• Molecular Weight: 29,936 Da
• NCBI Official Full Name: zinc finger protein SNAI2
• NCBI Official Synonym Full Names: snail family zinc finger 2
• NCBI Official Symbol: Snai2
• NCBI Official Synonym Symbols: Slug
• NCBI Protein Information: zinc finger protein SNAI2
• UniProt Protein Name: Zinc finger protein SNAI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: Snai2
• UniProt Synonym Gene Names: Slug; Slugh
• UniProt Entry Name: SNAI2_RAT

NCBI Description

transcriptional repressor; involved in epithelial-mesenchymal transition [RGD, Feb 2006]

Uniprot Description

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis ().