SMYD2 sirna

SMYD2 siRNA (Rat)

Cat. Num. AAA8241257

• Gene Names: Smyd2; HSKM-B
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMYD2; SMYD2 siRNA (Rat); N-lysine methyltransferase SMYD2; Histone methyltransferase SMYD2; SET and MYND domain-containing protein 2; SMYD2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SMYD2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 433

• Applicable Applications for SMYD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SMYD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMYD2 sirna

siRNA to inhibit SMYD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 281306785
• NCBI GeneID: 289372
• NCBI Accession #: NP_996733.1
• NCBI GenBank Nucleotide #: NM_206851.1
• UniProt Accession #: Q7M6Z3
• Molecular Weight: 49,648 Da
• NCBI Official Full Name: N-lysine methyltransferase SMYD2
• NCBI Official Synonym Full Names: SET and MYND domain containing 2
• NCBI Official Symbol: Smyd2
• NCBI Official Synonym Symbols: HSKM-B
• NCBI Protein Information: N-lysine methyltransferase SMYD2
• UniProt Protein Name: N-lysine methyltransferase SMYD2
• Protein Family: N-lysine methyltransferase
• UniProt Gene Name: Smyd2
• UniProt Entry Name: SMYD2_RAT

Uniprot Description

SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.

Protein type: Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: p53 binding; metal ion binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: histone methylation; negative regulation of cell proliferation; transcription, DNA-dependent; peptidyl-lysine di-methylation; heart development; peptidyl-lysine mono-methylation; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA damage response, signal transduction by p53 class mediator