SLC34A2 sirna

SLC34A2 siRNA (Rat)

Cat. Num. AAA8239725

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC34A2; SLC34A2 siRNA (Rat); Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na(+)-dependent phosphate cotransporter 2B; Sodium/phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2; rNaPi IIb; SLC34A2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC34A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 695

• Applicable Applications for SLC34A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC34A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC34A2 sirna

siRNA to inhibit SLC34A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758110
• NCBI GeneID: 84395
• NCBI Accession #: NP_445832.1
• NCBI GenBank Nucleotide #: NM_053380.2
• UniProt Accession #: Q9JJ09; Q8VI55
• Molecular Weight: 75,992 Da
• NCBI Official Full Name: sodium-dependent phosphate transport protein 2B
• NCBI Official Synonym Full Names: solute carrier family 34 (type II sodium/phosphate cotransporter), member 2
• NCBI Official Symbol: Slc34a2
• NCBI Protein Information: sodium-dependent phosphate transport protein 2B
• UniProt Protein Name: Sodium-dependent phosphate transport protein 2B
• Protein Family: Sodium-dependent phosphate transport protein
• UniProt Gene Name: Slc34a2
• UniProt Synonym Gene Names: Sodium-phosphate transport protein 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; rNaPi IIb
• UniProt Entry Name: NPT2B_RAT

NCBI Description

may act as a sodium dependent phosphate transporter; may provide inorganic phosphate for the synthesis of lung surfactant [RGD, Feb 2006]

Uniprot Description

SLC34A2: May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli. Defects in SLC34A2 are a cause of pulmonary alveolar microlithiasis (PALM). Pulmonary alveolar microlithiasis is a rare disease characterized by the deposition of calcium phosphate microliths throughout the lungs. Most patients are asymptomatic for several years or even for decades and generally, the diagnosis is incidental to clinical investigations unrelated to the disease. Cases with early onset or rapid progression are rare. A 'sandstorm-appearing' chest roentgenogram is a typical diagnostic finding. The onset of this potentially lethal disease varies from the neonatal period to old age and the disease follows a long-term, progressive course, resulting in a slow deterioration of lung functions. Pulmonary alveolar microlithiasis is a recessive monogenic disease with full penetrance. A chromosomal aberration involving SLC34A2 is found in a non-small cell lung cancer cell line. Results in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity. Belongs to the SLC34A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: microvillus membrane; brush border membrane; apical plasma membrane; integral to membrane; brush border; vesicle

Molecular Function: protein domain specific binding; sodium ion binding; phosphate binding; sodium-dependent phosphate transmembrane transporter activity; sodium:phosphate symporter activity

Biological Process: cellular phosphate ion homeostasis; phosphate transport; response to estrogen stimulus; in utero embryonic development; response to fructose stimulus; response to estradiol stimulus; aging