Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant MYH4, Human)
Mouse anti-Human Myosin Heavy Chain 4 (MYH4) Monoclonal Antibody | anti-MYH4 antibody
Monoclonal Antibody to Myosin Heavy Chain 4, Skeletal Muscle (MYH4)
Gene Names
MYH4; MYH2B; MyHC-2B; MyHC-IIb
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Myosin Heavy Chain 4 (MYH4); Monoclonal Antibody; Monoclonal Antibody to Myosin Heavy Chain 4; Skeletal Muscle (MYH4); MYH2B; Myosin heavy chain 2b; Myosin heavy chain; skeletal muscle; fetal; anti-MYH4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C3
Specificity
The antibody is a mouse monoclonal antibody raised against MYH4. It has been selected for its ability to recognize MYH4 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Leu1633~Leu1875
Sequence Length
1939
Applicable Applications for anti-MYH4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:500-2000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant MYH4, Human)
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant MYH4, Human)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin-4
NCBI Official Synonym Full Names
myosin heavy chain 4
NCBI Official Symbol
MYH4
NCBI Official Synonym Symbols
MYH2B; MyHC-2B; MyHC-IIb
NCBI Protein Information
myosin-4
UniProt Protein Name
Myosin-4
Protein Family
UniProt Gene Name
MYH4
UniProt Synonym Gene Names
MyHC-2b; MyHC-IIb
UniProt Entry Name
MYH4_HUMAN
Uniprot Description
MYH4: Muscle contraction.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: sarcomere; myofibril; muscle myosin complex
Molecular Function: calmodulin binding; microfilament motor activity; double-stranded RNA binding; actin binding; ATP binding
Biological Process: muscle contraction; actin filament-based movement; metabolic process; response to activity; muscle filament sliding
Research Articles on MYH4
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Product Notes
The MYH4 myh4 (Catalog #AAA2111299) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Myosin Heavy Chain 4, Skeletal Muscle (MYH4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin Heavy Chain 4 (MYH4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:500-2000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the MYH4 myh4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Leu1633~Le u1875. It is sometimes possible for the material contained within the vial of "Myosin Heavy Chain 4 (MYH4), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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