SLC27A5 sirna

SLC27A5 siRNA (Rat)

Cat. Num. AAA8207102

• Gene Names: Slc27a5; BAL; rBAL-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC27A5; SLC27A5 siRNA (Rat); ACSB; FATP5; VLACSR; Bile acyl-CoA synthetase; BACS; Bile acid-CoA ligase; BA-CoA ligase; BAL; Cholate--CoA ligase; Fatty acid transport protein 5; FATP-5; Solute carrier family 27 member 5; Very long-chain acyl-CoA synthetase-related protein; VLACS-related; SLC27A5 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC27A5 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 690

• Applicable Applications for SLC27A5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC27A5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC27A5 sirna

siRNA to inhibit SLC27A5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13162326
• NCBI GeneID: 79111
• NCBI Accession #: NP_077057.1
• NCBI GenBank Nucleotide #: NM_024143.2
• UniProt Accession #: Q9ES38
• Molecular Weight: 76,266 Da
• NCBI Official Full Name: bile acyl-CoA synthetase
• NCBI Official Synonym Full Names: solute carrier family 27 (fatty acid transporter), member 5
• NCBI Official Symbol: Slc27a5
• NCBI Official Synonym Symbols: BAL; rBAL-1
• NCBI Protein Information: bile acyl-CoA synthetase
• UniProt Protein Name: Bile acyl-CoA synthetase
• Protein Family: Bile acyl-CoA synthetase
• UniProt Gene Name: Slc27a5
• UniProt Synonym Gene Names: Acsb; Fatp5; Vlacsr; BACS; BA-CoA ligase; BAL; FATP-5; VLACS-related; VLACSR
• UniProt Entry Name: S27A5_RAT

NCBI Description

catalyzes the first of two steps in the conjugation of bile acids with the amino acids glycine and taurine [RGD, Feb 2006]

Uniprot Description

SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: Ligase; EC 6.2.1.7; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum

Cellular Component: protein complex; intracellular membrane-bound organelle; endoplasmic reticulum; basal plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: cholate-CoA ligase activity; fatty acid transporter activity; protein complex binding; very-long-chain-fatty-acid-CoA ligase activity; ATP binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: bile acid biosynthetic process; bile acid metabolic process; very-long-chain fatty acid metabolic process; ketone body biosynthetic process; long-chain fatty acid metabolic process; triacylglycerol mobilization; plasma membrane long-chain fatty acid transport; fatty acid transport