SLC27A2 sirna

SLC27A2 siRNA (Rat)

Cat. Num. AAA8203607

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC27A2; SLC27A2 siRNA (Rat); ACSVL1; FACVL1; FATP2; VLACS; VLCS; Very long-chain acyl-CoA synthetase; Fatty acid transport protein 2; FATP-2; Fatty-acid-coenzyme A ligase. very long-chain 1; Long-chain-fatty-acid--CoA ligase; Solute carrier family 27 member 2; THCA-CoA ligase; Very long-chain-fatty-acid-CoA ligase; SLC27A2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC27A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 620

• Applicable Applications for SLC27A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC27A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC27A2 sirna

siRNA to inhibit SLC27A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929034
• NCBI GeneID: 65192
• NCBI Accession #: NP_113924.1
• NCBI GenBank Nucleotide #: NM_031736.1
• UniProt Accession #: P97524
• Molecular Weight: 70,694 Da
• NCBI Official Full Name: very long-chain acyl-CoA synthetase
• NCBI Official Synonym Full Names: solute carrier family 27 (fatty acid transporter), member 2
• NCBI Official Symbol: Slc27a2
• NCBI Protein Information: very long-chain acyl-CoA synthetase
• UniProt Protein Name: Very long-chain acyl-CoA synthetase
• Protein Family: Very long-chain acyl-CoA synthetase
• UniProt Gene Name: Slc27a2
• UniProt Synonym Gene Names: Acsvl1; Facvl1; Fatp2; Vlacs; Vlcs; VLACS; VLCS; FATP-2
• UniProt Entry Name: S27A2_RAT

NCBI Description

has very long-chain acyl-CoA synthetase activity; may play a role in fatty acid metabolism [RGD, Feb 2006]

Uniprot Description

SLC27A2: Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes (By similarity)

Protein type: Membrane protein, integral; EC 6.2.1.3; Membrane protein, multi-pass; Ligase

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; integral to peroxisomal membrane; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; endoplasmic reticulum lumen; integral to membrane; integral to endoplasmic reticulum membrane; peroxisome

Molecular Function: protein binding; enzyme binding; fatty acid transporter activity; very-long-chain-fatty-acid-CoA ligase activity; phytanate-CoA ligase activity; ATP binding; receptor binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation; very-long-chain fatty acid metabolic process; very-long-chain fatty acid catabolic process; long-chain fatty acid metabolic process; fatty acid transport; fatty acid alpha-oxidation