SCAP sirna

SCAP siRNA (Rat)

Cat. Num. AAA8208507

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCAP; SCAP siRNA (Rat); Sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage-activating protein; SCAP sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SCAP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1276

• Applicable Applications for SCAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SCAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCAP sirna

siRNA to inhibit SCAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 155369287
• NCBI GeneID: 301024
• NCBI Accession #: NP_001094436.1
• NCBI GenBank Nucleotide #: NM_001100966.1
• UniProt Accession #: A2RRU4
• Molecular Weight: 139,499 Da
• NCBI Official Full Name: sterol regulatory element-binding protein cleavage-activating protein
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: Scap
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: Scap
• UniProt Synonym Gene Names: SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_RAT

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; protein complex; intracellular membrane-bound organelle; integral to membrane

Molecular Function: protein complex binding; cholesterol binding

Biological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; regulation of cholesterol biosynthetic process; regulation of fatty acid metabolic process; response to hypoxia; SREBP-mediated signaling pathway; aging; response to insulin stimulus