RET sirna

RET siRNA (Rat)

Cat. Num. AAA8212276

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RET; RET siRNA (Rat); Proto-oncogene tyrosine-protein kinase receptor Ret; RET sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RET siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1115

• Applicable Applications for RET sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RET gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RET sirna

siRNA to inhibit RET expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158534062
• NCBI GeneID: 24716
• NCBI Accession #: NP_036775.2
• NCBI GenBank Nucleotide #: NM_012643.2
• UniProt Accession #: G3V9H8; Q9EPA1; Q9EPC3
• Molecular Weight: 119,791 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform a
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: Ret
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Family: Retbindin
• UniProt Gene Name: Ret
• UniProt Entry Name: RET_RAT

NCBI Description

may play a role in excretory system and enteric nervous system development; human homolog is associated with Hirschsprung disease and Multiple Endocrine Neoplasia type 2 [RGD, Feb 2006]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Ret family

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; intracellular; receptor complex; lipid raft

Molecular Function: transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: regulation of cell adhesion; caspase activation; response to drug; nervous system development; positive regulation of neuron maturation; peptidyl-tyrosine phosphorylation; positive regulation of cell size; membrane protein proteolysis; positive regulation of transcription, DNA-dependent; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; response to pain; regulation of axonogenesis; neuron maturation; enteric nervous system development; neuron differentiation; retina development in camera-type eye; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; homophilic cell adhesion; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration