RAPGEF2 sirna

RAPGEF2 siRNA (Rat)

Cat. Num. AAA8216913

• Gene Names: Rapgef2; PDZ-GEF1; RA-GEF-1; nRap GEP
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAPGEF2; RAPGEF2 siRNA (Rat); PDZGEF1; Rap guanine nucleotide exchange factor 2; Cyclic nucleotide ras GEF; CNrasGEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA-GEF-1; Ras/Rap1-associating GEF-1; RAPGEF2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RAPGEF2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,496

• Applicable Applications for RAPGEF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RAPGEF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAPGEF2 sirna

siRNA to inhibit RAPGEF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157818583
• NCBI GeneID: 310533
• NCBI Accession #: NP_001101154.1
• NCBI GenBank Nucleotide #: NM_001107684.1
• UniProt Accession #: F1M386; D3ZD17
• Molecular Weight: 166,388 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 2
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 2
• NCBI Official Symbol: Rapgef2
• NCBI Official Synonym Symbols: PDZ-GEF1; RA-GEF-1; nRap GEP
• NCBI Protein Information: rap guanine nucleotide exchange factor 2
• UniProt Protein Name: Rap guanine nucleotide exchange factor 2
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: Rapgef2
• UniProt Synonym Gene Names: Pdzgef1; CNrasGEF; nRap GEP; PDZ-GEF1
• UniProt Entry Name: RPGF2_RAT

Uniprot Description

RAPGEF2: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. Interacts with MAGI2. Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.

Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Ras

Cellular Component: neuron projection; tight junction; protein complex; intercellular junction; cytosol; cell soma; membrane; perinuclear region of cytoplasm; endocytic vesicle; late endosome; apical plasma membrane; cytoplasm; plasma membrane; synapse; intracellular

Molecular Function: Rap guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; beta-1 adrenergic receptor binding; WW domain binding; cGMP binding; cAMP binding; protein kinase binding; Ras guanyl-nucleotide exchange factor activity; PDZ domain binding

Biological Process: blood vessel development; positive regulation of protein binding; brain-derived neurotrophic factor receptor signaling pathway; ventricular system development; neuron migration; regulation of synaptic plasticity; negative regulation of dendrite morphogenesis; microvillus biogenesis; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; regulation of small GTPase mediated signal transduction; cAMP-mediated signaling; neuropeptide signaling pathway; forebrain neuron development; positive regulation of protein kinase activity; small GTPase mediated signal transduction; negative regulation of melanin biosynthetic process; neurite development; Rap protein signal transduction; positive regulation of GTPase activity