PROM2 sirna

PROM2 siRNA (Rat)

Cat. Num. AAA8200018

• Gene Names: Prom2; Trprp; Promrp; Prom-rp
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PROM2; PROM2 siRNA (Rat); PROML2; TRPRP; Prominin-2; PROM-2; Prominin-like protein 2; rPROML2; Testosterone-regulated prominin-related protein; PROM2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PROM2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 886

• Applicable Applications for PROM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PROM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PROM2 sirna

siRNA to inhibit PROM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20302010
• NCBI GeneID: 192211
• NCBI Accession #: NP_620212.1
• NCBI GenBank Nucleotide #: NM_138857.1
• UniProt Accession #: Q8CJ52; Q8R4B6
• Molecular Weight: 92,841 Da
• NCBI Official Full Name: prominin-2
• NCBI Official Synonym Full Names: prominin 2
• NCBI Official Symbol: Prom2
• NCBI Official Synonym Symbols: Trprp; Promrp; Prom-rp
• NCBI Protein Information: prominin-2
• UniProt Protein Name: Prominin-2
• Protein Family: Prominin
• UniProt Gene Name: Prom2
• UniProt Synonym Gene Names: Proml2; Trprp; PROM-2; rPROML2
• UniProt Entry Name: PROM2_RAT

NCBI Description

polytopic membrane glycoprotein structurally related to prominin-1; may function in plasma membrane microdomains [RGD, Feb 2006]

Uniprot Description

PROM2: a multi-pass membrane protein of the prominin family that binds cholesterol. Localizes to the apical and basolateral membranes of epithelial cells. Present in saliva within small membrane particles. Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines. Three alternatively spliced human isoforms have been described.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: cell surface; microvillus; basolateral plasma membrane; integral to plasma membrane; integral to membrane; nuclear envelope; lipid raft; cilium; cell projection; microvillus membrane; apical plasma membrane; plasma membrane; cytoplasmic vesicle

Molecular Function: transmembrane receptor activity; growth factor activity; cholesterol binding

Biological Process: positive regulation of cell projection organization and biogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of pinocytosis; signal transduction