PRKAR1A sirna

PRKAR1A siRNA (Rat)

Cat. Num. AAA8221502

• Gene Names: Prkar1a; RIIA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRKAR1A; PRKAR1A siRNA (Rat); cAMP-dependent protein kinase type I-alpha regulatory subunit; PRKAR1A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PRKAR1A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 381

• Applicable Applications for PRKAR1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PRKAR1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRKAR1A sirna

siRNA to inhibit PRKAR1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981396
• NCBI GeneID: 25725
• NCBI Accession #: NP_037313.1
• NCBI GenBank Nucleotide #: NM_013181.1
• UniProt Accession #: P09456
• Molecular Weight: 43,095 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, alpha
• NCBI Official Symbol: Prkar1a
• NCBI Official Synonym Symbols: RIIA
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: Prkar1a
• UniProt Entry Name: KAP0_RAT

NCBI Description

regulatory subunit of cAMP-dependent protein kinase (PKA); negatively regulates meiosis [RGD, Feb 2006]

Uniprot Description

PKAR1A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Interacts with RFC2: the complex may be involved in cell survival. Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1). CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.

Protein type: Protein kinase, regulatory subunit

Cellular Component: protein complex; membrane; cytoplasm; plasma membrane; neuromuscular junction; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function: ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: mesoderm formation; heart development; negative regulation of meiosis; negative regulation of protein kinase activity; female meiosis; cardiac muscle cell proliferation; sarcomere organization; regulation of protein kinase activity