POLD1 sirna

POLD1 siRNA (Rat)

Cat. Num. AAA8222686

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLD1; POLD1 siRNA (Rat); DNA polymerase delta catalytic subunit; POLD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: POLD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1103

• Applicable Applications for POLD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat POLD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLD1 sirna

siRNA to inhibit POLD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11067381
• NCBI GeneID: 59294
• NCBI Accession #: NP_067694.1
• NCBI GenBank Nucleotide #: NM_021662.2
• UniProt Accession #: O54747
• Molecular Weight: 123,601 Da
• NCBI Official Full Name: DNA polymerase delta catalytic subunit
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: Pold1
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: Pold1
• UniProt Entry Name: DPOD1_RAT

NCBI Description

has DNA polymerase and 3'exonuclease activities; plays roles in DNA replication, base excision repair, and other types of DNA repair [RGD, Feb 2006]

Uniprot Description

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.