PICK1 sirna

PICK1 siRNA (Rat)

Cat. Num. AAA8232645

• Gene Names: Pick1; Prkcabp
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PICK1; PICK1 siRNA (Rat); PRKCABP; PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein; PICK1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PICK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 416

• Applicable Applications for PICK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PICK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PICK1 sirna

siRNA to inhibit PICK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758204
• NCBI GeneID: 84591
• NCBI Accession #: NP_445912.1
• NCBI GenBank Nucleotide #: NM_053460.1
• UniProt Accession #: Q9EP80; Q546X4; Q925D1
• Molecular Weight: 46,606 Da
• NCBI Official Full Name: PRKCA-binding protein
• NCBI Official Synonym Full Names: protein interacting with PRKCA 1
• NCBI Official Symbol: Pick1
• NCBI Official Synonym Symbols: Prkcabp
• NCBI Protein Information: PRKCA-binding protein
• UniProt Protein Name: PRKCA-binding protein
• Protein Family: PRKCA-binding protein
• UniProt Gene Name: Pick1
• UniProt Synonym Gene Names: Prkcabp
• UniProt Entry Name: PICK1_RAT

NCBI Description

PDZ domain containing protein; interacts with C-terminal domain of many central nervous system receptor proteins; may have an important role in signal transduction during synaptic transmission [RGD, Feb 2006]

Uniprot Description

PICK1: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel.

Protein type: Ligand, receptor tyrosine kinase; Cell development/differentiation; Adaptor/scaffold

Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; neuron projection; cytoskeleton; protein complex; mitochondrion; perinuclear region of cytoplasm; dendrite; postsynaptic density; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; protein domain specific binding; ephrin receptor binding; metabotropic glutatmate receptor binding; metal ion binding; ATPase activity; receptor tyrosine kinase binding; actin filament binding; GTPase binding; protein binding; enzyme binding; G-protein-coupled receptor binding; protein kinase C binding; kinase activity; receptor binding

Biological Process: glial cell development; dopamine transport; cellular response to glucose starvation; monoamine transport; positive regulation of receptor internalization; protein kinase C activation; protein amino acid phosphorylation; receptor clustering; phosphorylation; protein targeting