PDZK1 sirna

PDZK1 siRNA (Rat)

Cat. Num. AAA8205623

• Gene Names: Pdzk1; Clamp
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDZK1; PDZK1 siRNA (Rat); CLAMP; DIPHOR1; NHERF3; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; NHERF-3; C-terminal-linking and modulating protein; Dietary Pi-regulated RNA-1; Diphor-1; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3; PDZK1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PDZK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 523

• Applicable Applications for PDZK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDZK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDZK1 sirna

siRNA to inhibit PDZK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13928998
• NCBI GeneID: 65144
• NCBI Accession #: NP_113900.1
• NCBI GenBank Nucleotide #: NM_031712.1
• UniProt Accession #: Q9JJ40; O35234; Q6AZ21
• Molecular Weight: 56,800 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• NCBI Official Synonym Full Names: PDZ domain containing 1
• NCBI Official Symbol: Pdzk1
• NCBI Official Synonym Symbols: Clamp
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Protein Family: PDZK1-interacting protein
• UniProt Gene Name: Pdzk1
• UniProt Synonym Gene Names: Clamp; Diphor1; Nherf3; NHERF-3; NaPi-Cap1
• UniProt Entry Name: NHRF3_RAT

NCBI Description

binds the N-terminal PDZ domain of scavenger receptor class B type I; may play a role in intracellular transport of cholesterol esters [RGD, Feb 2006]

Uniprot Description

PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.

Protein type: Adaptor/scaffold

Cellular Component: microvillus membrane; brush border membrane; cytoplasm; apical plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; scavenger receptor binding; protein complex binding; PDZ domain binding

Biological Process: cholesterol transport; intracellular transport; transport; carnitine transport