PCNA sirna

PCNA siRNA (Rat)

Cat. Num. AAA8221098

• Gene Names: Pcna; PCNAR; Pcna/cyclin
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PCNA; PCNA siRNA (Rat); Proliferating cell nuclear antigen; Cyclin; PCNA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PCNA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for PCNA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PCNA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PCNA sirna

siRNA to inhibit PCNA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11693142
• NCBI GeneID: 25737
• NCBI Accession #: NP_071776.1
• NCBI GenBank Nucleotide #: NM_022381.3
• UniProt Accession #: P04961
• Molecular Weight: 28,749 Da
• NCBI Official Full Name: proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: Pcna
• NCBI Official Synonym Symbols: PCNAR; Pcna/cyclin
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: Pcna
• UniProt Synonym Gene Names: PCNA
• UniProt Entry Name: PCNA_RAT

NCBI Description

cell cycle protein that may contribute to the repair of DNA damage and to cell proliferation caused by exposure to pulmonary toxicants; may influence cell survival in ischaemically compromised tissue [RGD, Feb 2006]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: Cell cycle regulation; DNA replication

Cellular Component: nucleoplasm; nuclear lamina; centrosome; PCNA complex; nuclear replication fork; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; replication fork; intracellular; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; estrogen receptor binding; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding; transcription factor binding

Biological Process: base-excision repair, gap-filling; bypass DNA synthesis; mismatch repair; response to cadmium ion; epithelial cell differentiation; positive regulation of deoxyribonuclease activity; heart development; negative regulation of transcription from RNA polymerase II promoter; response to lipid; leading strand elongation; regulation of DNA replication