PCBD1 sirna

PCBD1 siRNA (Rat)

Cat. Num. AAA8216823

• Gene Names: Pcbd1; Pcd; Phs; DCoH; Pcbd; TCF1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PCBD1; PCBD1 siRNA (Rat); DCOH; PCBD; Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; PCBD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PCBD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 104

• Applicable Applications for PCBD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PCBD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PCBD1 sirna

siRNA to inhibit PCBD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 56090297
• NCBI GeneID: 29700
• NCBI Accession #: NP_001007602.1
• NCBI GenBank Nucleotide #: NM_001007601.2
• UniProt Accession #: P61459; P70519; P80095; Q9D930
• Molecular Weight: 12,000 Da
• NCBI Official Full Name: pterin-4-alpha-carbinolamine dehydratase
• NCBI Official Synonym Full Names: pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
• NCBI Official Symbol: Pcbd1
• NCBI Official Synonym Symbols: Pcd; Phs; DCoH; Pcbd; TCF1
• NCBI Protein Information: pterin-4-alpha-carbinolamine dehydratase
• UniProt Protein Name: Pterin-4-alpha-carbinolamine dehydratase
• Protein Family: Pterin-4-alpha-carbinolamine dehydratase
• UniProt Gene Name: Pcbd1
• UniProt Synonym Gene Names: Dcoh; Pcbd; PHS; DCoH; Dimerization cofactor of HNF1; PCD
• UniProt Entry Name: PHS_RAT

NCBI Description

This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. [provided by RefSeq, Jan 2014]

Uniprot Description

PCBD1: Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. Defects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D); also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients. Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Protein type: Lyase; Oxidoreductase; Transcription, coactivator/corepressor; EC 4.2.1.96

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; 4-alpha-hydroxytetrahydrobiopterin dehydratase activity; transcription coactivator activity; phenylalanine 4-monooxygenase activity

Biological Process: L-phenylalanine metabolic process; tetrahydrobiopterin biosynthetic process; transcription, DNA-dependent; protein heterooligomerization; regulation of protein homodimerization activity; positive regulation of transcription, DNA-dependent; protein homotetramerization