NTRK2 sirna

NTRK2 siRNA (Rat)

Cat. Num. AAA8235963

• Gene Names: Ntrk2; Tkrb; trkB; TRKB1; trk-B; RATTRKB1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NTRK2; NTRK2 siRNA (Rat); TRKB; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Trk-B; NTRK2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NTRK2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 474

• Applicable Applications for NTRK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NTRK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NTRK2 sirna

siRNA to inhibit NTRK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 253683488
• NCBI GeneID: 25054
• NCBI Accession #: NP_001156640.1
• NCBI GenBank Nucleotide #: NM_001163168.2
• UniProt Accession #: Q63604; Q63605; Q63606
• Molecular Weight: 52,971 Da
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor isoform 2
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: Ntrk2
• NCBI Official Synonym Symbols: Tkrb; trkB; TRKB1; trk-B; RATTRKB1
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: Ntrk2
• UniProt Synonym Gene Names: Trkb; Trk-B
• UniProt Entry Name: NTRK2_RAT

NCBI Description

receptor for brain-derived neurotrophic factor (BDNF) [RGD, Feb 2006]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Trk family

Cellular Component: cell surface; rough endoplasmic reticulum; cell; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; terminal button; excitatory synapse; perikaryon; cytosol; Golgi membrane; postsynaptic membrane; growth cone; cell soma; axon; cytoplasm; presynaptic active zone; plasma membrane; endosome membrane; intracellular; nerve terminal; endosome; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; transmembrane receptor protein tyrosine kinase activity; neurotrophin receptor activity; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; retinal rod cell development; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; regulation of dendrite development; neuron migration; regulation of neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; regulation of metabolic process; inflammatory response; vasculogenesis; neuromuscular junction development; central nervous system neuron development; aging; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; peripheral nervous system neuron development; long-term memory; glutamate secretion; regulation of MAPKKK cascade; positive regulation of axonogenesis; cerebral cortex development