NR4A3 sirna

NR4A3 siRNA (Rat)

Cat. Num. AAA8236748

• Gene Names: Nr4a3; NOR-2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NR4A3; NR4A3 siRNA (Rat); NOR1; Nuclear receptor subfamily 4 group A member 3; Neuron-derived orphan receptor 1/2; Nuclear hormone receptor NOR-1/NOR-2; NR4A3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NR4A3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 430

• Applicable Applications for NR4A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NR4A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NR4A3 sirna

siRNA to inhibit NR4A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13994173
• NCBI GeneID: 58853
• NCBI Accession #: NP_113816.1
• NCBI GenBank Nucleotide #: NM_031628.1
• UniProt Accession #: P51179; Q2XPY2; Q63516; Q9QWQ3
• Molecular Weight: 46,240 Da
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 3
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 3
• NCBI Official Symbol: Nr4a3
• NCBI Official Synonym Symbols: NOR-2
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 3
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 3
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: Nr4a3
• UniProt Synonym Gene Names: Nor1
• UniProt Entry Name: NR4A3_RAT

NCBI Description

nuclear orphan receptor; involved in regulating transcription [RGD, Feb 2006]

Uniprot Description

NR4A3: Binds to the B1A response-element. Defects in NR4A3 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving NR4A3 is found in patients with Erwing sarcoma. Translocation t(9;22)(q22-31;q11-12) with EWSR1. A chromosomal aberration involving NR4A3 is a cause of a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with TAF2N. Belongs to the nuclear hormone receptor family. NR4 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Nuclear receptor; DNA-binding

Cellular Component: transcription factor complex; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; axon guidance; organ regeneration; inner ear morphogenesis; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; hippocampus development; semicircular canal morphogenesis; positive regulation of cell cycle; mesoderm formation; response to hydrogen peroxide; vestibular reflex; adult behavior; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; neuromuscular process controlling balance; negative regulation of apoptosis