NOTCH2 sirna

NOTCH2 siRNA (Rat)

Cat. Num. AAA8231544

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOTCH2; NOTCH2 siRNA (Rat); Neurogenic locus notch homolog protein 2; Notch 2; NOTCH2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NOTCH2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2471

• Applicable Applications for NOTCH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NOTCH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOTCH2 sirna

siRNA to inhibit NOTCH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20138817
• NCBI GeneID: 29492
• UniProt Accession #: Q9QW30
• Molecular Weight: 265,370 Da
• NCBI Official Full Name: Neurogenic locus notch homolog protein 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: Notch2
• NCBI Protein Information: neurogenic locus notch homolog protein 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch2
• UniProt Synonym Gene Names: Notch 2
• UniProt Entry Name: NOTC2_RAT

NCBI Description

transmembrane protein that may be involved in cellular interactions that specify cell fate [RGD, Feb 2006]

Uniprot Description

Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; membrane; cell; integral to plasma membrane; extracellular region; plasma membrane; nucleus; cytosol; receptor complex; cilium

Molecular Function: NF-kappaB binding; enzyme binding; calcium ion binding; receptor activity

Biological Process: wound healing; positive regulation of osteoclast differentiation; positive regulation of apoptosis; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; tissue regeneration; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; heart looping; cell cycle arrest; cell growth; positive regulation of BMP signaling pathway; placenta development; regulation of developmental process; embryonic limb morphogenesis; Notch signaling pathway; transcription, DNA-dependent; in utero embryonic development; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; positive regulation of Ras protein signal transduction; determination of left/right symmetry