NME2 sirna

NME2 siRNA (Rat)

Cat. Num. AAA8205963

• Gene Names: Nme2; NDKB; nm23-2; p18-12d
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NME2; NME2 siRNA (Rat); Nucleoside diphosphate kinase B; NDK B; NDP kinase B; Histidine protein kinase NDKB; P18; NME2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NME2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 152

• Applicable Applications for NME2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NME2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NME2 sirna

siRNA to inhibit NME2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55926145
• NCBI GeneID: 83782
• NCBI Accession #: NP_114021.2
• NCBI GenBank Nucleotide #: NM_031833.2
• UniProt Accession #: P19804
• Molecular Weight: 17,283 Da
• NCBI Official Full Name: nucleoside diphosphate kinase B
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 2
• NCBI Official Symbol: Nme2
• NCBI Official Synonym Symbols: NDKB; nm23-2; p18-12d
• NCBI Protein Information: nucleoside diphosphate kinase B
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: Nme2
• UniProt Synonym Gene Names: NDK B; NDP kinase B
• UniProt Entry Name: NDKB_RAT

NCBI Description

kinase involved in synthesis of nucleoside triphosphates [RGD, Feb 2006]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; EC 2.7.4.6; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; EC 2.7.13.3; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Cellular Component: ruffle; focal adhesion; mitochondrion; perinuclear region of cytoplasm; lamellipodium; mitochondrial membrane; cytoplasm; plasma membrane; intermediate filament; nucleus; cytosol; myelin sheath

Molecular Function: protein serine/threonine kinase activity; enzyme binding; protein histidine kinase activity; metal ion binding; intermediate filament binding; nucleoside diphosphate kinase activity; drug binding; fatty acid binding; ATP binding

Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; UTP biosynthetic process; protein amino acid autophosphorylation; negative regulation of myeloid leukocyte differentiation; nucleoside diphosphate phosphorylation; protein oligomerization; regulation of apoptosis; CTP biosynthetic process; nucleoside triphosphate biosynthetic process; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; regulation of epidermis development; negative regulation of apoptosis; positive regulation of keratinocyte differentiation