Human Chordin Like Protein 1 (CHRDL1) ELISA Kit | CHRDL1 elisa kit

Human Chordin Like Protein 1 (CHRDL1) ELISA Kit

Cat. Num. AAA2705870

• Gene Names: CHRDL1; CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• Reactivity: Human
• Synonyms: Chordin Like Protein 1 (CHRDL1); Human Chordin Like Protein 1 (CHRDL1) ELISA Kit; NRLN1; CHL; VOPT; Ventroptin; Neuralin-1; Neurogenesin-1; CHRDL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chordin Like Protein 1 (CHRDL1).; No significant cross-reactivity or interference between Chordin Like Protein 1 (CHRDL1) and analogues was observed.
• Sequence Length: 456

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRDL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin Like Protein 1 (CHRDL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin Like Protein 1 (CHRDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin Like Protein 1 (CHRDL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chordin Like Protein 1 (CHRDL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 221316561
• NCBI GeneID: 91851
• NCBI Accession #: NP_001137453.1
• NCBI GenBank Nucleotide #: NM_001143981.1
• UniProt Accession #: Q9BU40; Q539E4; Q9Y3H7; B1AKD0; B4DMP3; D3DUY6; E9PGS5
• Molecular Weight: 52,026 Da
• NCBI Official Full Name: chordin-like protein 1 isoform 1
• NCBI Official Synonym Full Names: chordin like 1
• NCBI Official Symbol: CHRDL1
• NCBI Official Synonym Symbols: CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• NCBI Protein Information: chordin-like protein 1
• UniProt Protein Name: Chordin-like protein 1
• Protein Family: Chordin-like protein
• UniProt Gene Name: CHRDL1
• UniProt Synonym Gene Names: NRLN1

NCBI Description

This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation (). May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.