MYO5B sirna

MYO5B siRNA (Rat)

Cat. Num. AAA8233632

• Gene Names: Myo5b; Myr6
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO5B; MYO5B siRNA (Rat); Unconventional myosin-Vb; Myosin heavy chain myr 6; MYO5B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MYO5B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1846

• Applicable Applications for MYO5B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MYO5B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO5B sirna

siRNA to inhibit MYO5B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393817
• NCBI GeneID: 25132
• NCBI Accession #: NP_058779.1
• NCBI GenBank Nucleotide #: NM_017083.1
• UniProt Accession #: P70569
• Molecular Weight: 213,721 Da
• NCBI Official Full Name: unconventional myosin-Vb
• NCBI Official Synonym Full Names: myosin Vb
• NCBI Official Symbol: Myo5b
• NCBI Official Synonym Symbols: Myr6
• NCBI Protein Information: unconventional myosin-Vb
• UniProt Protein Name: Unconventional myosin-Vb
• Protein Family: Unconventional myosin
• UniProt Gene Name: Myo5b
• UniProt Entry Name: MYO5B_RAT

NCBI Description

3 sequence has high similarity to a putative mouse glutamic acid decarboxylase [RGD, Feb 2006]

Uniprot Description

MYO5B: May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis. Defects in MYO5B are a cause of diarrhea type 2 (DIAR2). DIAR2 is characterized by onset of intractable life- threatening watery diarrhea during infancy. Two forms are recognized: early-onset MVID with diarrhea beginning in the neonatal period, and late-onset, with first symptoms appearing after 3 or 4 months of life.

Protein type: Motility/polarity/chemotaxis; Motor

Cellular Component: neuron projection; protein complex; cell soma; apical cortex; perinuclear region of cytoplasm; dendritic spine; myosin complex

Molecular Function: calmodulin binding; ionotropic glutamate receptor binding; protein binding; motor activity; actin binding; ATP binding; Rab GTPase binding

Biological Process: vesicle-mediated transport; protein transport; regulation of protein localization; metabolic process; endosome localization