MET sirna

MET siRNA (Rat)

Cat. Num. AAA8238489

• Gene Names: Met; Hgfr
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MET; MET siRNA (Rat); Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MET siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1382

• Applicable Applications for MET sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MET gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MET sirna

siRNA to inhibit MET expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13928700
• NCBI GeneID: 24553
• NCBI Accession #: NP_113705.1
• NCBI GenBank Nucleotide #: NM_031517.2
• UniProt Accession #: P97523; P97579; Q63119; Q63964
• Molecular Weight: 153,941 Da
• NCBI Official Full Name: hepatocyte growth factor receptor
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: Met
• NCBI Official Synonym Symbols: Hgfr
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: Metaxin
• UniProt Gene Name: Met
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_RAT

NCBI Description

hepatocyte growth factor receptor; functions as a heterodimeric tyrosine kinase [RGD, Feb 2006]

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.1; TK group; Met family

Cellular Component: extracellular space; neuron projection; cell surface; dendrite; postsynaptic density; integral to membrane; excitatory synapse; postsynaptic membrane; membrane; cell soma; cytoplasm; basal plasma membrane; plasma membrane

Molecular Function: phospholipase binding; protein heterodimerization activity; protein-tyrosine kinase activity; protein complex binding; beta-catenin binding; phosphoinositide 3-kinase binding; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding; protein kinase activity

Biological Process: lactation; response to peptide hormone stimulus; central nervous system neuron differentiation; muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; myoblast proliferation; neuron migration; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to organic cyclic substance; positive chemotaxis; pancreas development; response to wounding; endothelial cell morphogenesis; oligodendrocyte development; placenta development; regulation of synaptic transmission; response to drug; skeletal muscle development; positive regulation of mitosis; male gonad development; muscle cell migration; myotube differentiation; cell aging; hepatocyte growth factor receptor signaling pathway; positive regulation of dendrite morphogenesis; liver development; positive regulation of peptidyl-serine phosphorylation; sperm motility; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; response to acidity; brain development; regulation of excitatory postsynaptic membrane potential; positive regulation of DNA replication