Human TEAD4 (Transcriptional enhancer factor TEF-3) ELISA Kit | TEAD4 elisa kit

Human TEAD4 (Transcriptional enhancer factor TEF-3) ELISA Kit

Cat. Num. AAA7612198

• Gene Names: TEAD4; TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B
• Reactivity: Human
• Synonyms: TEAD4 (Transcriptional enhancer factor TEF-3); Human TEAD4 (Transcriptional enhancer factor TEF-3) ELISA Kit; Transcriptional enhancer factor TEF-3; TEA domain family member 4; TEAD-4; Transcription factor 13-like 1; Transcription factor RTEF-1; RTEF1; TCF13L1; TEF3; TEAD4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TEAD4. No significant cross-reactivity or interference between TEAD4 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TEAD4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 215274211
• NCBI GeneID: 7004
• UniProt Accession #: Q15561; Q6MZR9; Q8NEV5; Q92883; Q96BK2; H0Y308
• Molecular Weight: 434
• NCBI Official Full Name: Transcriptional enhancer factor TEF-3
• NCBI Official Synonym Full Names: TEA domain family member 4
• NCBI Official Symbol: TEAD4
• NCBI Official Synonym Symbols: TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B
• NCBI Protein Information: transcriptional enhancer factor TEF-3; transcription factor RTEF-1; transcription factor 13-like 1; transcriptional enhancer factor 3; related transcription enhancer factor 1B; transcriptional enhancer factor 1-related
• UniProt Protein Name: Transcriptional enhancer factor TEF-3
• Protein Family: Transcriptional enhancer factor
• UniProt Gene Name: TEAD4
• UniProt Synonym Gene Names: RTEF1; TCF13L1; TEF3; TEAD-4
• UniProt Entry Name: TEAD4_HUMAN

NCBI Description

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TEAD4: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12p13.3-p13.2

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; muscle development; gene expression; skeletal development