MAX sirna

MAX siRNA (Rat)

Cat. Num. AAA8206899

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAX; MAX siRNA (Rat); Protein max; Myc-associated factor X; MAX sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAX siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 151

• Applicable Applications for MAX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAX sirna

siRNA to inhibit MAX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11559988
• NCBI GeneID: 60661
• NCBI Accession #: NP_071546.1
• NCBI GenBank Nucleotide #: NM_022210.1
• UniProt Accession #: P52164
• Molecular Weight: 17,199 Da
• NCBI Official Full Name: protein max
• NCBI Official Synonym Full Names: MYC associated factor X
• NCBI Official Symbol: Max
• NCBI Protein Information: protein max
• UniProt Protein Name: Protein max
• Protein Family: Maximin
• UniProt Gene Name: Max
• UniProt Entry Name: MAX_RAT

NCBI Description

human homolog is a transcriptional regulator that binds Myc [RGD, Feb 2006]

Uniprot Description

MAX: a transcription factor. Forms a DNA- binding protein complex with MYC or MAD. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. CPG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC/MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3-K9 histone methyltransferase activity. Three alternatively spliced isoforms have been reported.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; neuron apoptosis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; retina development in camera-type eye; protein complex assembly; response to axon injury; cellular response to starvation; response to organic nitrogen; response to insulin stimulus