MAP3K1 sirna

MAP3K1 siRNA (Rat)

Cat. Num. AAA8225280

• Gene Names: Map3k1; Mekk1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP3K1; MAP3K1 siRNA (Rat); MEKK; MEKK1; Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; MAP3K1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAP3K1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1493

• Applicable Applications for MAP3K1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAP3K1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP3K1 sirna

siRNA to inhibit MAP3K1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758760
• NCBI GeneID: 116667
• NCBI Accession #: NP_446339.1
• NCBI GenBank Nucleotide #: NM_053887.1
• UniProt Accession #: Q62925
• Molecular Weight: 161,315 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 1
• NCBI Official Synonym Full Names: mitogen activated protein kinase kinase kinase 1
• NCBI Official Symbol: Map3k1
• NCBI Official Synonym Symbols: Mekk1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 1
• Protein Family: MAP3K12-binding inhibitory protein
• UniProt Gene Name: Map3k1
• UniProt Synonym Gene Names: Mekk; Mekk1; MEK kinase 1; MEKK 1
• UniProt Entry Name: M3K1_RAT

NCBI Description

kinase that has E3 ubiquitin ligase to mediate ubiquitination and degradation of ERK1/2 [RGD, Feb 2006]

Uniprot Description

MEKK1: a protein kinase of the STE11 family. Phosphorylates and activates MKK4 and -7, which in turn activate JNK1, -2 and -3. Contains 1 RING-type zinc finger. The RING finger domain of MEKK1 exhibits E3 ubiquitin ligase activity toward c-Jun. c-Jun is downregulated in response to osmotic stress via ubiquitination-mediated degradation by the RING finger domain of MEKK1. Overexpressed/overactivated in multiple tumor types. The Mek1/2 inhibitor U0126 blocks export of influenza viral particles and has been suggested as an antiviral treatment. Single mutations at the same point of each gene also associated with cardiofaciocutaneous syndrome, (also linked with Braf and Kras mutations), displaying morphological, cardiac and mental defects. Inhibitors: U0126, CI-1040/PD184352, PD-0325901 (Phase I cancer), ARRY-142886 (Phase 1, cancer).

Protein type: Ligase; Ubiquitin conjugating system; Protein kinase, STE; Kinase, protein; Ubiquitin ligase; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Cellular Component: cytoskeleton; membrane; cytoplasm; intracellular

Molecular Function: protein serine/threonine kinase activity; sphingolipid binding; JUN kinase binding; protein binding; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity; zinc ion binding; mitogen-activated protein kinase kinase binding; ubiquitin-protein ligase activity; mitogen-activated protein kinase binding; JUN kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; negative regulation of actin filament bundle formation; camera-type eye development; protein polyubiquitination; activation of MAPKK activity; wound healing; positive regulation of viral transcription; positive regulation of apoptosis; protein amino acid autophosphorylation; protein ubiquitination during ubiquitin-dependent protein catabolic process; MAPKKK cascade; response to osmotic stress; regulation of cell migration; protein amino acid phosphorylation; protein oligomerization; activation of JNK activity; positive regulation of MAP kinase activity; apoptotic mitochondrial changes; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; transforming growth factor beta receptor signaling pathway; JNK cascade; positive regulation of transcription from RNA polymerase II promoter; activation of JNKK activity